I Congreso de Postgrado fcfm: ingeniería, ciencias e innovación

122 Santiago, 10 al 12 de agosto, 2022 BIOCONVERSION OF SEAWEED BIOMASS THROUGH AN ARTIFICIAL FUNGI CONSORTIUM INTO A NEW ALTERNATIVE PROTEIN AND FUNCTIONAL FOOD INGREDIENT Catalina Landeta 1 *, Nicolás Salas 1 , Lhaís Araújo-Caldas 2 , Allison Leyton 3 , Patricia Sartorelli 2 , María Elena Lienqueo 1 1 Center for Biotechnology and Bioengineering (CeBiB), Department of Ch mical Engineering, Biotechnology, and Materials, University of Chile, Beauchef 851, Santiago, Chile. 2Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brazil. 3 Center for Biotechnology and Bioengineering (CeBiB), Center of Food Biotechnology and Bioseparations, BIOREN and Department of Chemical Engineering, Universidad de La Frontera, Francisco Salazar 01145, Temuco 4780000, Chile. *Email: cmlandeta@uc.cl ABSTRACT The stages of production of proteins of animal origin to satisfy human needs require abundant natural resources and their intake is associated with health problems. It is necessary to search for appropriate substitutes more sustainable and healthy. In this research, we obtained for the first time an alternative and functional protein rich in proteins (~48% DW), amino acids (~29% DW), and ß-D-glucans (~22% DW). This product is the result of en- hancing the nutritional value of the Chilean brown seaweed Durvillaea incurvata through a submerged fermenta- tion process with a unique GRAS artificial consortium of marine and terrestrial fungi. This artificial consortium is regarded as part of the solution to debottleneck the physiological limitations of mono-cultures bioprocesses, such as degrading complex carbon sources and efficient substrate utilization. The rationally designed consortium demonstrated properties that exceeded the monoculture properties, including a ~150% increase in productivity, a significant increase in total protein (~336%), amino acids (~245%), and ß-D-glucans (~ 100%). This product has all essential amino acids, low content of fatty acids (8,7%), good content of total dietary fiber (26.8%), high antioxidant activity (TEAC of 34 μM/g), no toxic metabolites, no heavy metals, and no pesticides. In this study, an untargeted metabolomics approach combined with multivariate statistical analysis and dereplication tech- niques aides by the GNPS Molecular Network was employed to screen the differential metabolites and to identify molecules with nutraceutical properties of the monoculture and of three artificial consortia designed. Thus, the results of principal component analysis revealed their distinct secondary (mono-cultive: PC1, 41.5%; co-cultive: PC2, 30.7%) metabolite patterns, and allowed to discriminate the chemical composition between co-culture and mono-culture. Among the screened 110-top differential metabolites, 22 nutraceutical compounds involving un- saturated fatty acids and fatty acid amides such and as linoleic acid, palmitamide, 13-docosenamide, 9-octadece- namide, among others showed higher content in co-cultive. In conclusion, the bioconversion of Durvillaea incur- vata through an artificial fungi consortium results in a product that has high-quality protein, great nutritional value, with potential prebiotic due to the presence of ß-D-glucans, and potential nutraceutical due to the produc- tion of unique bioactive compounds such as fatty acid amides with broad bioactivity. ACKNOWLEDGMENTS Centre for Biotechnology and Bioengineering – CeBiB – (Project FB-0001). REFERENCIAS [1] I. Ergal, O. Gräf, B. Hasibar, M. Steiner, S. Vukotic, Comm. Bio. 3, 1-12 (2020) I NNOVAC I ÓN E N T E CNO LOG Í A Y P ROC E SOS 10